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02-15-2010, 01:14 PM
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#11 (permalink) |
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Moderator
 
Join Date: Feb 2009
Location: Austin, Texas, USA
Posts: 696
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Wow, our first post from a veritable rock star of both the hobby and the science!
Welcome, Ghazanfar. We are certainly happy if not surprised to see you! If I may ask, what else do you tissue culture? Do you work out of your home or a lab? I presume your primary interest is in aquatic Aroids what with all the wonderful information you've given us over the years regarding Cryptocorynes and Lagenandras. Again, welcome and it is an honor to have such a person of distinction as a member. Sorry for gushing.... 
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02-15-2010, 01:53 PM
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#12 (permalink) |
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Junior Member
Join Date: Feb 2010
Posts: 8
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You're right, my current interest is primarily in aquatic aroids, mostly cryptocoryne and lagenandra, but I will be trying my hand at Anubias sp. soon as well. Other plants I've cultured include Styrogyne sp. and the obligatory African Violet.
My 'lab' is a spare room in my house that's rarely used for much for at all. No laminar flow hood YET  - using basic home tissue culture techniques with a good success rate.I've been meaning to take some videos and posted them up - hopefully I'll get around to that soon. However, I did just take some new pictures yesterday of some of the jars which I'll be posting on my blog this week - so keep an eye out for that. Always more than happy to share information and learn from others. |
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02-17-2010, 09:54 AM
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#14 (permalink) | |
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Junior Member
Join Date: Feb 2010
Posts: 8
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Quote:
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02-17-2010, 11:45 AM
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#16 (permalink) | |
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Junior Member
Join Date: Feb 2010
Posts: 8
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Quote:
It's fun to grow rare plants, and with some of these rare crypts, cultivation is quite difficult. The challenge appeals to me - the I love the plants too. I guess that explains why I'm addicted to crypts!
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02-17-2010, 05:06 PM
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#18 (permalink) |
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Aquatic Plant Enthusiast
Join Date: Feb 2009
Location: Gainesville, FL
Posts: 436
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Ghazanfar - I sent Dr Kane the link to your blog. He was very impressed with the detail of your documentation and pictures.
Back to my original topic - About 1/4 of my cultures are infected due to low surface sterilization technique due to this plant coming directly from my aquarium. I went with 5% and 10% bleach solutions. All heart cuttings have to be thrown out along with several bud tips due to fungal growth. I'm going in tomorrow to pull some of the non-infected buds and re-sterilize at 20% with longer agitation (normal procedure for terrestrial plants). Then I plan to remove the outer layer of cells and innoculate the bud meristem tissue by itself. Last edited by chillplants; 02-17-2010 at 05:08 PM. |
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02-18-2010, 09:27 AM
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#19 (permalink) | |
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Junior Member
Join Date: Feb 2010
Posts: 8
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Quote:
Interested in seeing how your re-sterilization works out - please keep us updated. |
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02-19-2010, 03:51 PM
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#20 (permalink) |
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Aquatic Plant Enthusiast
Join Date: Feb 2009
Location: Gainesville, FL
Posts: 436
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Originally I was going to bring the Erio to school and acclimitize it in the mist house for emmersed form, but the weather was too cold. I was also afraid the buds would flower once out of the water.
Sidenote - I did a 3 day blackout about a month ago to help with some BBA problems. Once I took the cover off the tank, my Erio started budding. I had about 20 buds by the time this project started. I re-sterilized 5 buds yesterday with 20% bleach solution and 10 min agitation. I tried to remove the outer tissue around the bud, but it was nearly impossible and the buds inside were somewhat gelatinous. I only have a couple of the original buds that are not infected along with some leaf tissue that I did not re-sterilize. Hopefully they will still be fungus free on Monday when I check on them again. |
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